Ca 2+ Influx Through Ca 2+ Channels in Rabbit Ventricular Myocytes During Action Potential Clamp
نویسندگان
چکیده
منابع مشابه
Ca Influx Through Ca Channels in Rabbit Ventricular Myocytes During Action Potential Clamp Influence of Temperature
Ca influx via Ca current (ICa) during the action potential (AP) was determined at 25°C and 35°C in isolated rabbit ventricular myocytes using AP clamp. Contaminating currents through Na and K channels were eliminated by using Naand K-free solutions, respectively. DIDS (0.2 mmol/L) was used to block Ca-activated chloride current (ICl(Ca)). When the sarcoplasmic reticulum (SR) was depleted of Ca ...
متن کاملCa(2+) influx through Ca(2+) channels in rabbit ventricular myocytes during action potential clamp: influence of temperature.
Ca(2+) influx via Ca(2+) current (I(Ca)) during the action potential (AP) was determined at 25 degrees C and 35 degrees C in isolated rabbit ventricular myocytes using AP clamp. Contaminating currents through Na(+) and K(+) channels were eliminated by using Na(+)- and K(+)-free solutions, respectively. DIDS (0.2 mmol/L) was used to block Ca(2+)-activated chloride current (I(Cl(Ca))). When the s...
متن کاملProfile of L-type Ca(2+) current and Na(+)/Ca(2+) exchange current during cardiac action potential in ventricular myocytes.
OBJECTIVE The L-type Ca(2+) current (I(Ca,L)) and the Na(+)/Ca(2+) exchange current (I(NCX)) are major inward currents that shape the cardiac action potential (AP). Previously, the profile of these currents during the AP was determined from voltage-clamp experiments that used Ca(2+) buffer. In this study, we aimed to obtain direct experimental measurement of these currents during cardiac AP wit...
متن کاملPredicting local SR Ca(2+) dynamics during Ca(2+) wave propagation in ventricular myocytes.
Of the many ongoing controversies regarding the workings of the sarcoplasmic reticulum (SR) in cardiac myocytes, two unresolved and interconnected topics are 1), mechanisms of calcium (Ca(2+)) wave propagation, and 2), speed of Ca(2+) diffusion within the SR. Ca(2+) waves are initiated when a spontaneous local SR Ca(2+) release event triggers additional release from neighboring clusters of SR r...
متن کاملCa Sparks in Rabbit Ventricular Myocytes Evoked by Action Potentials Involvement of Clusters of L-Type Ca Channels
It is not clear how many L-type Ca channels (LCCs) are required to ensure that a Ca spark is triggered during a normal mammalian action potential (AP). We investigated this in rabbit ventricular myocytes by examining both the properties of sparks evoked by APs and the activity of LCCs. We measured Ca sparks evoked by repeated APs with pipettes containing 2 mmol/L EGTA and single LCC activity in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Circulation Research
سال: 1999
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.85.6.e7